Journal: Experimental and Therapeutic Medicine

Article Title: Exosomes from Ub‑HBcAg‑overexpressing dendritic cells induce T‑lymphocyte differentiation and enhance cytotoxic T‑lymphocyte activity

doi: 10.3892/etm.2023.11866

Figure Lengend Snippet: Isolation and identification of Dexs-Ub-HBcAg. (A) Transmission electron microscopy of the mDexs ultrastructure. Scale bar, 200 nm. (B) Expression of the positive exosomal markers CD9, CD63 and TSG101, and of HBcAg was examined via the western blot analysis of Dexs lysates. (C) Size dispersion profile of mDexs evaluated using ZetaVIEW ® nanoparticle tracking analysis, indicating a size peak of 123.8 nm. Dexs, dendritic cell-derived exosomes; HBcAg, hepatitis B virus core antigen; Dexs-Ub-HBcAg, Dexs loaded with ubiquitinated HBcAg; Con, control; TSG101, tumor susceptibility gene 101.

Article Snippet: Subsequently, the membrane was blocked with 5% non-fat milk at room temperature for 1 h. The primary antibodies used were rabbit anti-mouse CD63 (1:500; cat. no. ab216130; Abcam), anti-CD9 (1:500; cat. no. ab92726; Abcam) and tumor susceptibility gene 101 (TSG101; 1:500; cat. no. 102286-T38; SinoBiological) monoclonal antibodies.

Techniques: Isolation, Transmission Assay, Electron Microscopy, Expressing, Western Blot, Dispersion, Derivative Assay, Virus, Control